Row 1:
 
Sunset Shimmer  
Twilight Sparkle  
Rainbow Dash  
Pinkie Pie  
Fluttershy  
Applejack  
Rarity  
Sci-Twi  
Spike
 
Row 2:
 
Principal Celestia  
Vice Principal Luna  
Adagio Dazzle  
Aria Blaze  
Sonata Dusk  
Flash Sentry  
Apple Bloom  
Sweetie Belle  
Scootaloo
 
Row 3:
 
Miss Cheerilee  
Big Macintosh  
Granny Smith  
Diamond Tiara  
Silver Spoon  
Trixie Lulamoon  
Derpy Hands  
Vinyl Scratch  
Octavia Melody
 
Row 4:
 
Snips and Snails(A duo, like the Ice Climbers)  
Mrs. Cake  
Mr. Cake  
Lyra Heartstrings  
Bon Bon  
Maud Pie  
Bulk Biceps  
Blueberry Cake  
Normal Norman
 
Row 5:
 
Mystery Mint  
Starlight  
Tennis Match  
Sandalwood  
Cloudy Kicks  
Watermelody  
Photo Finish  
Pixel Pizzazz  
Violet Blurr
 
Row 6:
 
Principal Cinch  
Dean Cadance  
Shining Armor  
Mr. Cranky Doodle  
Lemon Zest  
Sour Sweet  
Sugarcoat  
Sunny Flare  
Indigo Zap
 
Row 7:
 
Flim Flam Brothers(work as a duo)  
Scribble Dee  
Micro Chips  
Wiz Kid  
Curly Winds  
Heath Burns  
Valhallen  
Crimson Napalm  
Nolan North
 
Row 8:
 
Scott Green  
Rose Heart  
Aqua Blossom  
Golden Hazel  
Sweet Leaf  
Velvet Sky  
Captain Planet  
Teddy  
Thunderbass
 
Bonus Characters:
 
Evil Sunset Shimmer  
Midnight Sparkle  
Blythe Baxter  
Optimus Prime  
Jem  
Angel Bunny  
Mii