Seven Virtues for Seven Heroes:
 
Twilight Sparkle: Chastity  
The state or practice of refraining from extramarital, or especially from all, sexual intercourse.
 
Rainbow Dash: Diligence  
Constant and earnest effort to accomplish what is undertaken;persistent exertion of body or mind.
 
Fluttershy: Kindness  
The quality of being friendly, generous, and considerate.
 
Pinkie Pie: Temperance  
To show moderation or self-restraint, especially in eating and drinking.
 
Rarity: Charity  
The voluntary showing generosity and helpfulness, especially toward the needy or suffering.
 
Applejack: Humility  
The quality or state of not thinking one is better than other people.
 
Spike: Patience  
The capacity to accept or tolerate delay, trouble, or suffering without getting angry or upset.